Isolation of Chondrocytes from Human Cartilage and Cultures in Monolayer and 3D

Rodrigo A Somoza; Jean F Welter

doi:10.1007/978-1-0716-1119-7_1

Isolation of Chondrocytes from Human Cartilage and Cultures in Monolayer and 3D

Methods Mol Biol. 2021:2245:1-12. doi: 10.1007/978-1-0716-1119-7_1.

Authors

Rodrigo A Somoza¹, Jean F Welter²

Affiliations

¹ Department of Biology, Skeletal Research Center, Case Center for Multimodal Evaluation of Engineered Cartilage, Case Western Reserve University, Cleveland, OH, USA.
² Department of Biology, Skeletal Research Center, Case Center for Multimodal Evaluation of Engineered Cartilage, Case Western Reserve University, Cleveland, OH, USA. [email protected].

PMID: 33315191
DOI: 10.1007/978-1-0716-1119-7_1

Abstract

Chondrocytes are the only cell type in cartilage. The dense cartilage extracellular matrix surrounding the chondrocytes makes isolating these cells a complex and lengthy task that subjects the cells to harsh conditions. Protocols to isolate expand and maintain these cells have been improved over the years, providing ways to obtain viable cells for tissue engineering and clinical applications. Here we describe a method to obtain populations of chondrocytes that are able to expand and maintain a native-like phenotype.

Keywords: Cartilage ECM; Human chondrocytes; Sequential digestion.

Publication types

Research Support, N.I.H., Extramural

MeSH terms

Cartilage, Articular / cytology*
Cell Culture Techniques*
Cell Separation* / methods
Cells, Cultured
Chondrocytes / cytology*
Humans
Spheroids, Cellular

Abstract

Publication types

MeSH terms

Grants and funding