In Vivo Administration of Therapeutic Antisense Oligonucleotides

Methods Mol Biol. 2021:2254:273-282. doi: 10.1007/978-1-0716-1158-6_17.

Abstract

With the rapid revolution in RNA/DNA sequencing technologies, it is evident that mammalian genomes express tens of thousands of long noncoding RNAs (lncRNAs). Since a large majority of lncRNAs have been functionally implicated in cancer development and progression, there is an increasing appreciation for the use of antisense oligonucleotide (ASO)-based therapies targeting lncRNAs in several cancers. Despite their great potential in therapeutic applications, their use is still limited due to cellular toxicity and shortcomings in achieving required stability in biological fluids and tissue uptake. To overcome these limitations, major changes in ASO chemistry have been introduced to generate second and third generation ASOs, including locked nucleic acids (LNA) technology. Here we describe two different LNA-ASO delivery approaches, a peritumoral administration and a systemic delivery in xenograft models of lung adenocarcinoma, that significantly reduced tumor growth without inducing toxicity.

Keywords: Antisense oligonucleotides; LNA-ASOs; Long noncoding RNAs; Lung cancer; Xenografts; lncRNAs.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • A549 Cells
  • Adenocarcinoma of Lung / genetics
  • Adenocarcinoma of Lung / therapy*
  • Animals
  • Cell Line, Tumor
  • Gene Expression Regulation, Neoplastic / drug effects
  • Humans
  • Lung Neoplasms / genetics
  • Lung Neoplasms / therapy*
  • Mice
  • Oligonucleotides, Antisense / administration & dosage*
  • Oligonucleotides, Antisense / pharmacology
  • Pilot Projects
  • RNA, Long Noncoding / genetics*
  • Xenograft Model Antitumor Assays

Substances

  • Oligonucleotides, Antisense
  • RNA, Long Noncoding