Complete mitochondrial genome of Tolypocladium sp. YFCC 1805002 isolated from Ophiocordyceps sinensis in Baima Snow Mountain, Southwestern China

Yanfang Liu; Dexiang Tang; Guodong Zhang; Mingxi Zhang; Yao Wang; Yuanbing Wang; Hong Yu

doi:10.1080/23802359.2019.1698338

Complete mitochondrial genome of Tolypocladium sp. YFCC 1805002 isolated from Ophiocordyceps sinensis in Baima Snow Mountain, Southwestern China

Mitochondrial DNA B Resour. 2019 Dec 13;5(1):171-173. doi: 10.1080/23802359.2019.1698338.

Authors

Yanfang Liu^{1

2}, Dexiang Tang^{1

2}, Guodong Zhang^{1

2}, Mingxi Zhang^{1

2}, Yao Wang^{1

2}, Yuanbing Wang^{1

2

3}, Hong Yu^{1

2}

Affiliations

¹ Yunnan Herbal Laboratory, School of Life Sciences, Yunnan University, Kunming, China.
² The International Joint Research Center for Sustainable Utilization of Cordyceps Bioresources in China and Southeast Asia, Yunnan University, Kunming, China.
³ The Research Center of Cordyceps Development and Utilization of Kunming, Yunnan Herbal Biotech Co. Ltd, Kunming, China.

Abstract

Tolypocladium sp. YFCC 1805002, an undescribed species isolated from natural Ophiocordyces sinensis in Baima Snow Mountain, Deqin County of Yunnan in Southwestern China, was sequenced on the Illumina sequencing platform. The complete mitochondrial genome of this fungus is a circular molecule of 46,466 bp in length, and this value is higher than its two relatives T. inflatum and T. ophioglossoides. The circular mitogenome contains 15 protein-coding genes (PCGs), a set of 25 transfer RNA (tRNA) genes, and 2 ribosomal RNA (rns and rnl) genes. The 15 protein-coding genes are atp6, 8-9, cob, cox1-3, nad1-6, nad4L and rps3. The lengths of 25 transfer RNA (tRNA) genes are ranging from 71 to 87 bp, and the sizes of rns and rnl are 1554 bp and 5931 bp, respectively. The overall base composition is 38.3% A, 35.6% T, 11.6% C, 14.5% G, with a low GC content of 26.1%. Phylogenetic analysis inferred from concatenated protein-coding genes of 51 taxa shows that the new species Tolypocladium sp. YFCC 1805002 is closely related to T. inflatum in the family Ophiocordycipitaceae with high credible support by Bayesian inference posterior probabilities (BI-PP = 100%). This study would facilitate the future research of genetics, evolution and medicine of cordycipitoid fungi.

Keywords: Mitochondrial genome; Tolypocladium; phylogenetic analysis.

Grants and funding

This work was jointly funded by the China Postdoctoral Science Foundation [2017M613017], and National Natural Science Foundation of China [31760011, 31870017], and Ministry of Science and Technology of the People's Republic of China [KC1610530], and Department of Science and Technology of Yunnan Province [2018IA075, 2018FY001(-006)], and Biodiversity Survey and Assessment Project of the Ministry of Ecology and Environment, China [2019HJ2096001006], and Yunnan University's Research Innovation Fund for Graduate Students [YDY17100].