Characterization of complete mitochondrial genome of Nemania diffusa (Xylariaceae, Xylariales) and its phylogenetic analysis

Dexiang Tang; Guodong Zhang; Yao Wang; Mingxi Zhang; Yuanbing Wang; Hong Yu

doi:10.1080/23802359.2019.1704665

Characterization of complete mitochondrial genome of Nemania diffusa (Xylariaceae, Xylariales) and its phylogenetic analysis

Mitochondrial DNA B Resour. 2020 Jan 8;5(1):459-460. doi: 10.1080/23802359.2019.1704665.

Authors

Dexiang Tang^{1

2}, Guodong Zhang^{1

2}, Yao Wang^{1

2}, Mingxi Zhang^{1

2}, Yuanbing Wang^{1

2

3}, Hong Yu^{1

2}

Affiliations

¹ Yunnan Herbal Laboratory, School of Life Sciences, Yunnan University, Kunming, China.
² The International Joint Research Center for Sustainable Utilization of Cordyceps Bioresources in China and Southeast Asia, Yunnan University, Kunming, China.
³ The Research Center of Cordyceps Development and Utilization of Kunming, Yunnan Herbal Biotech Co. Ltd, Kunming, China.

Abstract

Nemania diffusa is a parasitic fungus of many plant species and causes large economic losses to the forestry industry. In the present study, the complete mitochondrial genome of N. diffusa is first reported. The circular genome is 258,879 bp in length, containing 14 protein coding genes, 2 ribosomal RNA genes, 25 transfer RNA genes. The 258,879 bp long mtDNA of N. diffusa represents one of the largest sequenced fungal mitogenomes. The overall base composition is 34.4% A, 35.9% T, 14.0% G, 15.7% C and the content of GC is 29.7%. Phylogenetic analysis based on concatenated protein coding genes from 24 species in 8 orders was conducted using Bayesian inference (BI) method. Nemania diffusa is clustered in the order Xylariales and is more closely related to Annulohypoxylon stygium of Hypoxylaceae. This work facilitates the future study of molecular biology and evolution of xylariaceous fungi.

Keywords: Mitochondrial genome; Nemania diffusa; phylogenetic analysis.

Grants and funding

This work was funded by the China Postdoctoral Science Foundation [2017M613017], and the National Natural Science Foundation of China [31760011, 31870017], and the Department of Science and Technology of Yunnan Province [2018IA075, 2018FY001(-006)], and the Project of Yunnan University Service Yunnan Action Plan [KS161020], and the Biodiversity Survey and Assessment Project of the Ministry of Ecology and Environment, China [2019HJ2096001006].