Sulfonation and Antioxidative Evaluation of Polysaccharides from Pleurotus Mushroom and Streptococcus thermophilus Bacteria: A Review

Human beings are equipped with antioxidant defense systems to neutralize free radicals as free radicals could damage macromolecules, subsequently resulting in serious diseases. Researchers have been attracted to search for potential natural antioxidants to reduce oxidative damage. Pleurotus and Streptococcus thermophilus have been chosen as sources of sustainable bioactive compounds that have been consumed for thousands of years. Polysaccharides are important bioactive components produced by Pleurotus mushrooms and Streptococcus thermophilus bacteria. Additionally, there is a continued interest in sulfonation of crude polysaccharides from both sources, since sulfonation has been found to improve or create new bioactive properties in polysaccharides. Both crude and sulfated polysaccharides with good antioxidant capacities have great potential for the further development as commercial products. This review focuses on characterization, sulfonation methods, and antioxidant capacity evaluations of polysaccharides from Pleurotus and S. thermophilus. Common antioxidant capacity assays, including the mechanisms underlying each assay, and various experimental procedures are also discussed.