Comparative genomics with a multidrug-resistant Klebsiella pneumoniae isolate reveals the panorama of unexplored diversity in Northeast Brazil

Gene. 2021 Mar 10:772:145386. doi: 10.1016/j.gene.2020.145386. Epub 2020 Dec 26.

Abstract

The emergence of community acquired infections increases the public health concern on K. pneumoniae and closely related bacteria among which antimicrobial resistance spreads. We report a multidrug-resistant K. pneumoniae isolate, B31, of a patient infected in the community and admitted to an intensive care unit in Northeast Brazil. Antimicrobial susceptibility and genome information were thoroughly investigated to characterize B31 in front of 172 sequenced strains of different countries. Assigned to the Sequence Type 15, which is globally spread, B31 presented extended spectrum beta-lactamase, tigecycline and ciprofloxacin resistance. Genome sequencing revealed most resistance genes being carried by plasmids with high dissemination potential. The absence of main virulence factors, like yersiniabactin and colibactin, apparently suggests a mild pathogenic strain which, on the contrary, persisted and caused severe infection in a previously healthy patient. The present study contributes to unveil the unclear genomic scenario of virulent and multidrug-resistant K. pneumoniae in Brazil.

Keywords: Antimicrobial Evaluation; Hospital Emergency Care; Klebsiella pneumoniae; Multilocus Sequence Type; Phylogenomics; Virulence Genes.

MeSH terms

  • Adult
  • Ciprofloxacin / pharmacology
  • Community-Acquired Infections / microbiology*
  • Drug Resistance, Multiple, Bacterial*
  • Female
  • Genome, Bacterial
  • High-Throughput Nucleotide Sequencing
  • Humans
  • Klebsiella Infections / microbiology*
  • Klebsiella pneumoniae / classification*
  • Klebsiella pneumoniae / genetics
  • Multilocus Sequence Typing
  • Plasmids / genetics
  • Tigecycline / pharmacology
  • Whole Genome Sequencing / methods*

Substances

  • Ciprofloxacin
  • Tigecycline