Induction of alarmin S100A8/A9 mediates activation of aberrant neutrophils in the pathogenesis of COVID-19

Cell Host Microbe. 2021 Feb 10;29(2):222-235.e4. doi: 10.1016/j.chom.2020.12.016. Epub 2020 Dec 26.

Abstract

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic poses an unprecedented public health crisis. Evidence suggests that SARS-CoV-2 infection causes dysregulation of the immune system. However, the unique signature of early immune responses remains elusive. We characterized the transcriptome of rhesus macaques and mice infected with SARS-CoV-2. Alarmin S100A8 was robustly induced in SARS-CoV-2-infected animal models as well as in COVID-19 patients. Paquinimod, a specific inhibitor of S100A8/A9, could rescue the pneumonia with substantial reduction of viral loads in SARS-CoV-2-infected mice. Remarkably, Paquinimod treatment resulted in almost 100% survival in a lethal model of mouse coronavirus infection using the mouse hepatitis virus (MHV). A group of neutrophils that contributes to the uncontrolled pathological damage and onset of COVID-19 was dramatically induced by coronavirus infection. Paquinimod treatment could reduce these neutrophils and regain anti-viral responses, unveiling key roles of S100A8/A9 and aberrant neutrophils in the pathogenesis of COVID-19, highlighting new opportunities for therapeutic intervention.

Keywords: Paquinimod; S100A8/A9; SARS-CoV-2; aberrant neutrophils.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alarmins / pharmacology*
  • Animals
  • Antiviral Agents / pharmacology*
  • COVID-19 / metabolism
  • COVID-19 / virology
  • COVID-19 Drug Treatment*
  • Disease Models, Animal
  • Female
  • Humans
  • Macaca mulatta
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Neutrophils / drug effects*
  • Neutrophils / metabolism
  • SARS-CoV-2 / drug effects*
  • Transcriptome
  • Viral Load

Substances

  • Alarmins
  • Antiviral Agents