Cardiomyocytes stimulate angiogenesis after ischemic injury in a ZEB2-dependent manner

Nat Commun. 2021 Jan 4;12(1):84. doi: 10.1038/s41467-020-20361-3.

Abstract

The disruption in blood supply due to myocardial infarction is a critical determinant for infarct size and subsequent deterioration in function. The identification of factors that enhance cardiac repair by the restoration of the vascular network is, therefore, of great significance. Here, we show that the transcription factor Zinc finger E-box-binding homeobox 2 (ZEB2) is increased in stressed cardiomyocytes and induces a cardioprotective cross-talk between cardiomyocytes and endothelial cells to enhance angiogenesis after ischemia. Single-cell sequencing indicates ZEB2 to be enriched in injured cardiomyocytes. Cardiomyocyte-specific deletion of ZEB2 results in impaired cardiac contractility and infarct healing post-myocardial infarction (post-MI), while cardiomyocyte-specific ZEB2 overexpression improves cardiomyocyte survival and cardiac function. We identified Thymosin β4 (TMSB4) and Prothymosin α (PTMA) as main paracrine factors released from cardiomyocytes to stimulate angiogenesis by enhancing endothelial cell migration, and whose regulation is validated in our in vivo models. Therapeutic delivery of ZEB2 to cardiomyocytes in the infarcted heart induces the expression of TMSB4 and PTMA, which enhances angiogenesis and prevents cardiac dysfunction. These findings reveal ZEB2 as a beneficial factor during ischemic injury, which may hold promise for the identification of new therapies.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Movement / genetics
  • Cell Proliferation / genetics
  • Dependovirus / metabolism
  • Gene Expression Regulation
  • Humans
  • Ischemia / genetics
  • Ischemia / pathology*
  • Mice
  • Mice, Knockout
  • Models, Biological
  • Myocardial Infarction / genetics
  • Myocardial Infarction / pathology
  • Myocytes, Cardiac / metabolism*
  • Myocytes, Cardiac / pathology
  • Neovascularization, Physiologic* / genetics
  • Protein Precursors / genetics
  • Protein Precursors / metabolism
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Thymosin / analogs & derivatives
  • Thymosin / genetics
  • Thymosin / metabolism
  • Zinc Finger E-box Binding Homeobox 2 / genetics
  • Zinc Finger E-box Binding Homeobox 2 / metabolism*

Substances

  • Protein Precursors
  • RNA, Messenger
  • ZEB2 protein, mouse
  • Zinc Finger E-box Binding Homeobox 2
  • prothymosin alpha
  • thymosin beta(4)
  • Thymosin