Abstract
Tri-nitrilotriacetic acid (NTA)-based fluorescent probes were developed and used to image His-tagged-labelled outer membrane protein C (His-OmpC) in live Escherichia coli. One of these probes was designed to light up upon binding, which provided the means to assess changes in the His-OmpC expression levels by taking a simple fluorescence spectrum.
MeSH terms
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Binding Sites
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Escherichia coli / genetics
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Escherichia coli / metabolism
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Escherichia coli Proteins / genetics
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Escherichia coli Proteins / metabolism*
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Fluorescent Dyes / chemistry*
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Gene Expression Regulation, Bacterial / physiology*
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Gene Expression*
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Membrane Proteins / chemistry
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Membrane Proteins / genetics
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Membrane Proteins / metabolism*
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Molecular Probes / chemistry*
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Molecular Structure
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Protein Binding
Substances
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Escherichia coli Proteins
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Fluorescent Dyes
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Membrane Proteins
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Molecular Probes