Common genes and pathways involved in the response to stressful stimuli by astrocytes: A meta-analysis of genome-wide expression studies

Genomics. 2021 Mar;113(2):669-680. doi: 10.1016/j.ygeno.2021.01.008. Epub 2021 Jan 21.

Abstract

Astrocytes play pivotal roles in the brain and they become reactive under stress conditions. Here, we carried out, for the first time, an integrative meta-analysis of genome-wide expression profiling of astrocytes from human and mouse exposed to different stressful stimuli (hypoxia, infections by virus and bacteria, cytokines, ethanol, among others). We identified common differentially expressed genes and pathways in human and murine astrocytes. Our results showed that astrocytes induce expression of genes associated with stress response and immune system regulation when they are exposed to stressful stimuli, whereas genes related to neurogenesis are found as downregulated. Several of the identified genes showed to be important hubs in the protein-protein interaction analysis (TRAF2, CDC37 and PAX6). This work demonstrates that despite astrocytes are highly heterogeneous and complex, there are common gene expression signatures that can be triggered under distinct detrimental stimuli, which opens an opportunity for exploring other possible markers of reactivity.

Keywords: Astrocytes; Convergence analysis; Genome-wide expression profiling; Reactivity; Stress response.

Publication types

  • Meta-Analysis
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Astrocytes / metabolism*
  • Cell Cycle Proteins / genetics
  • Cell Cycle Proteins / metabolism
  • Chaperonins / genetics
  • Chaperonins / metabolism
  • Gene Regulatory Networks*
  • Humans
  • Mice
  • Neurogenesis
  • PAX6 Transcription Factor / genetics
  • PAX6 Transcription Factor / metabolism
  • Stress, Physiological*
  • TNF Receptor-Associated Factor 2 / genetics
  • TNF Receptor-Associated Factor 2 / metabolism
  • Transcriptome*

Substances

  • CDC37 protein, human
  • Cell Cycle Proteins
  • PAX6 Transcription Factor
  • PAX6 protein, human
  • PSMD2 protein, human
  • TNF Receptor-Associated Factor 2
  • Chaperonins