Protocol for isolation of spermatids from mouse testes

Chang Rok Kim; Taichi Noda; Yuki Okada; Masahito Ikawa; Sung Hee Baek

doi:10.1016/j.xpro.2020.100254

Protocol for isolation of spermatids from mouse testes

STAR Protoc. 2021 Jan 8;2(1):100254. doi: 10.1016/j.xpro.2020.100254. eCollection 2021 Mar 19.

Authors

Chang Rok Kim¹, Taichi Noda², Yuki Okada³, Masahito Ikawa^{2

4}, Sung Hee Baek¹

Affiliations

¹ Research Center for Epigenetic Code and Diseases, Department of Biological Sciences, Seoul National University, Seoul 08826, South Korea.
² Research Institute for Microbial Diseases, Osaka University, Osaka 565-0871, Japan.
³ Laboratory of Pathology and Development, Institute for Quantitative Biosciences, The University of Tokyo, Tokyo 113-0032, Japan.
⁴ The Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan.

Abstract

Post-meiotic spermatids become spermatozoa through developmental stages during spermiogenesis. Isolation of spermatid fractions is required to examine the change of protein expression during spermiogenesis. Here, we present a simple method to isolate spermatid fractions from mouse testes using unit gravity sedimentation in a BSA density gradient. Isolation of spermatid fractions can be used to analyze changes of transcript or protein during spermiogenesis. For complete details on the use and execution of this protocol, please refer to Kim et al. (2020).

Keywords: Cell Biology; Cell isolation; Model Organisms.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Separation*
Male
Mice
Spermatids / cytology*
Spermatids / metabolism
Spermatogenesis
Testis / cytology*
Testis / metabolism