Optical technologies for the detection of viruses like COVID-19: Progress and prospects

Biosens Bioelectron. 2021 Apr 15:178:113004. doi: 10.1016/j.bios.2021.113004. Epub 2021 Jan 16.

Abstract

The outbreak of life-threatening pandemic like COVID-19 necessitated the development of novel, rapid and cost-effective techniques that facilitate detection of viruses like SARS-CoV-2. The presently popular approach of a collection of samples using the nasopharyngeal swab method and subsequent detection of RNA using the real-time polymerase chain reaction suffers from false-positive results and a longer diagnostic time scale. Alternatively, various optical techniques namely optical sensing, spectroscopy, and imaging shows a great promise in virus detection. Herein, a comprehensive review of the various photonics technologies employed for virus detection, particularly the SARS-CoV family, is discussed. The state-of-art research activities in utilizing the photonics tools such as near-infrared spectroscopy, Fourier transform infrared spectroscopy, Raman spectroscopy, fluorescence-based techniques, super-resolution microscopy, surface plasmon resonance-based detection, for virus detection accounted extensively with an emphasis on coronavirus detection. Further, an account of emerging photonics technologies of SARS-CoV-2 detection and future possibilities is also explained. The progress in the field of optical techniques for virus detection unambiguously show a great promise in the development of rapid photonics-based devices for COVID-19 detection.

Keywords: COVID-19; Optical imaging; Optical spectroscopy; Photonics; Surface plasmon resonance; Virus.

Publication types

  • Review

MeSH terms

  • Biosensing Techniques / methods
  • COVID-19 / diagnosis*
  • COVID-19 / virology*
  • COVID-19 Testing / methods*
  • COVID-19 Testing / trends
  • Humans
  • Molecular Diagnostic Techniques
  • Nucleic Acid Amplification Techniques
  • Optical Phenomena
  • Pandemics
  • SARS-CoV-2 / genetics
  • SARS-CoV-2 / isolation & purification*
  • Spectroscopy, Fourier Transform Infrared
  • Spectrum Analysis, Raman
  • Surface Plasmon Resonance

Supplementary concepts

  • LAMP assay