Complete Genome Sequences of 12 Quinolone-Resistant Escherichia coli Strains Containing qnrS1 Based on Hybrid Assemblies

Håkon Kaspersen; Thomas H A Haverkamp; Hanna Karin Ilag; Øivind Øines; Camilla Sekse; Jannice Schau Slettemeås

doi:10.1128/MRA.01190-20

Complete Genome Sequences of 12 Quinolone-Resistant Escherichia coli Strains Containing qnrS1 Based on Hybrid Assemblies

Microbiol Resour Announc. 2021 Jan 28;10(4):e01190-20. doi: 10.1128/MRA.01190-20.

Authors

Håkon Kaspersen¹, Thomas H A Haverkamp², Hanna Karin Ilag², Øivind Øines¹, Camilla Sekse¹, Jannice Schau Slettemeås³

Affiliations

¹ Department of Animal Health and Food Safety, Norwegian Veterinary Institute, Oslo, Norway.
² Department of Analysis and Diagnostics, Norwegian Veterinary Institute, Oslo, Norway.
³ Department of Animal Health and Food Safety, Norwegian Veterinary Institute, Oslo, Norway [email protected].

Abstract

In total, 12 quinolone-resistant Escherichia coli (QREC) strains containing qnrS1 were submitted to long-read sequencing using a FLO-MIN106 flow cell on a MinION device. The long reads were assembled with short reads (Illumina) and analyzed using the MOB-suite pipeline. Six of these QREC genome sequences were closed after hybrid assembly.