Characterization of fibrillar collagen isoforms in infarcted mouse hearts using second harmonic generation imaging

Sushant P Sahu; Qianglin Liu; Alisha Prasad; Syed Mohammad Abid Hasan; Qun Liu; Maria Ximena Bastidas Rodriguez; Orna Mukhopadhyay; David Burk; Joseph Francis; Supratik Mukhopadhyay; Xing Fu; Manas Ranjan Gartia

doi:10.1364/BOE.410347

Characterization of fibrillar collagen isoforms in infarcted mouse hearts using second harmonic generation imaging

Biomed Opt Express. 2020 Dec 23;12(1):604-618. doi: 10.1364/BOE.410347. eCollection 2021 Jan 1.

Authors

Affiliations

¹ Department of Chemistry, University of Louisiana at Lafayette, Lafayette, LA 70504, USA.
² LSU AgCenter, School of Animal Sciences, Louisiana State University, Baton Rouge, LA 70803, USA.
³ Department of Mechanical and Industrial Engineering, Louisiana State University, Baton Rouge, LA 70803, USA.
⁴ Department of Computer Science, Louisiana State University, Baton Rouge, LA 70803, USA.
⁵ Baton Rouge Magnet High School, Baton Rouge, LA 70806, USA.
⁶ Shared Instrumentation Facility and Pennington Biomedical Research Center, Baton Rouge, LA 70808, USA.
⁷ Comparative Biomedical Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA 70803, USA.
⁸ [email protected].
⁹ [email protected].

Abstract

We utilized collagen specific second harmonic generation (SHG) signatures coupled with correlative immunofluorescence imaging techniques to characterize collagen structural isoforms (type I and type III) in a murine model of myocardial infarction (MI). Tissue samples were imaged over a four week period using SHG, transmitted light microscopy and immunofluorescence imaging using fluorescently-labeled collagen antibodies. The post-mortem cardiac tissue imaging using SHG demonstrated a progressive increase in collagen deposition in the left ventricle (LV) post-MI. We were able to monitor structural morphology and LV remodeling parameters in terms of extent of LV dilation, stiffness and fiber dimensions in the infarcted myocardium.

Abstract

Grants and funding