Protocol for a mouse CNS demyelination model induced by a combination of HSV-1 and IL-2

STAR Protoc. 2021 Jan 19;2(1):100287. doi: 10.1016/j.xpro.2020.100287. eCollection 2021 Mar 19.

Abstract

Multiple sclerosis (MS) is an autoimmune demyelinating disease of the central nervous system. Although viruses have been suggested to be a contributing environmental factor, conventional experimental MS mouse models do not account for this aspect. Here, we describe a mouse model to induce and evaluate demyelinating disease with both a viral and an immune component via ocular infection with a recombinant herpes simplex virus expressing murine interleukin-2. For complete details on the use and execution of this protocol, please refer to Hirose et al. (2020).

Keywords: Model organisms; Neuroscience.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Cell Line
  • Disease Models, Animal
  • Herpes Simplex / genetics
  • Herpes Simplex / immunology*
  • Herpesvirus 1, Human / immunology*
  • Interleukin-2 / adverse effects*
  • Interleukin-2 / immunology
  • Interleukin-2 / pharmacology
  • Mice
  • Mice, Knockout
  • Multiple Sclerosis / genetics
  • Multiple Sclerosis / immunology*
  • Rabbits

Substances

  • Interleukin-2