Visualization of cortical microtubule networks in plant cells by live imaging and immunostaining

Fei Du; Feng Zhao; Jan Traas; Yuling Jiao

doi:10.1016/j.xpro.2021.100301

Visualization of cortical microtubule networks in plant cells by live imaging and immunostaining

STAR Protoc. 2021 Jan 23;2(1):100301. doi: 10.1016/j.xpro.2021.100301. eCollection 2021 Mar 19.

Authors

Fei Du¹, Feng Zhao², Jan Traas², Yuling Jiao^{1

3}

Affiliations

¹ State Key Laboratory of Plant Genomics and National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing 100101, China.
² Laboratoire Reproduction et Développement des Plantes, Univ Lyon, ENS de Lyon, UCB Lyon 1, CNRS, INRAE, Inria, 69342 Lyon, France.
³ University of Chinese Academy of Sciences, Beijing 100049, China.

Abstract

Cortical microtubules (CMTs) play pivotal roles during plant cell growth and division. The organization of CMTs undergoes important changes during different cellular and developmental processes. Here, we describe two methods for the visualization of CMT organization in plant cells using confocal laser scanning microscopy. CMT networks in the outer tissue layers can be directly visualized by live imaging of a fluorescent reporter line, and a protocol combining sectioning and immunostaining is applied for visualization of CMTs throughout tissues. For complete details on the use and execution of this protocol, please refer to Zhao et al. (2020).

Keywords: Cell biology; Microscopy; Model organisms.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Arabidopsis / cytology
Microscopy, Confocal / methods*
Microtubules / chemistry
Microtubules / metabolism*
Optical Imaging / methods*
Plant Cells / metabolism
Plants
Solanum lycopersicum / metabolism