Antitumor Effect of the Ethanolic Extract from Seeds of Euphorbia lathyris in Colorectal Cancer

Nutrients. 2021 Feb 9;13(2):566. doi: 10.3390/nu13020566.

Abstract

The seeds of Euphorbia lathyris have been used in traditional medicine to treat various medical conditions. However, neither all of their active biocompounds nor the molecular mechanisms underlying their therapeutic effects have been described. A new ethanolic extract of defatted flour from mature seeds of Euphorbia lathyris showed a high total polyphenol content and significant antioxidant activity. Chromatographic analysis showed that esculetin, euphorbetin, gaultherin, and kaempferol-3-rutinoside were the most abundant polyphenolic bioactive compounds. Antiproliferative assays showed a high and selective antitumor activity against colon cancer cell lines (T84 and HCT-15). In addition, a significant antiproliferative activity against glioblastoma multiforme cells was also demonstrated. Its mechanism of action to induce cell death was mediated by the overexpression of caspases 9, 3, and 8, and by activation of autophagy. Interestingly, a reduction in the migration capacity of colon cancer cells and a significant antiangiogenic effect on human umbilical vein endothelial cells were also demonstrated. Finally, the extract significantly reduced the subpopulations of cancer stem cells. This extract could be the basis to develop new therapeutic strategies for the treatment of colon cancer, although further experiments will be necessary to determine its in vivo effects.

Keywords: Euphorbia lathyris; antiangiogenic effect; apoptosis; cancer stem cells; colon cancer; ethanolic extract.

MeSH terms

  • Adenocarcinoma / pathology
  • Antineoplastic Agents, Phytogenic
  • Antioxidants / analysis
  • Apoptosis / drug effects
  • Cell Line, Tumor
  • Cell Proliferation / drug effects*
  • Colonic Neoplasms / drug therapy
  • Colonic Neoplasms / pathology*
  • Ethanol
  • Euphorbia / chemistry*
  • Glioblastoma / pathology
  • Human Umbilical Vein Endothelial Cells
  • Humans
  • Neovascularization, Physiologic / drug effects
  • Plant Extracts / pharmacology*
  • Plant Extracts / therapeutic use
  • Polyphenols / analysis
  • Seeds / chemistry*

Substances

  • Antineoplastic Agents, Phytogenic
  • Antioxidants
  • Plant Extracts
  • Polyphenols
  • Ethanol