FISH in Suspension or in Adherent Cells

Francesca Di Pippo; Diogo Queirós; Joana Pereira; Paulo C Lemos; Luísa S Serafim; Simona Rossetti

doi:10.1007/978-1-0716-1115-9_4

FISH in Suspension or in Adherent Cells

Methods Mol Biol. 2021:2246:51-67. doi: 10.1007/978-1-0716-1115-9_4.

Authors

Francesca Di Pippo¹, Diogo Queirós², Joana Pereira², Paulo C Lemos³, Luísa S Serafim⁴, Simona Rossetti¹

Affiliations

¹ Water Research Institute C.N.R., Monterotondo, Italy.
² CICECO - Aveiro Institute of Materials, Chemistry Department, University of Aveiro, Aveiro, Portugal.
³ LAQV, REQUIMTE, Chemistry Department, Faculty of Science and Technology, University NOVA of Lisbon, Caparica, Portugal.
⁴ CICECO - Aveiro Institute of Materials, Chemistry Department, University of Aveiro, Aveiro, Portugal. [email protected].

PMID: 33576982
DOI: 10.1007/978-1-0716-1115-9_4

Abstract

Fluorescence in situ hybridization (FISH) enables the detection and enumeration of microorganisms in a diversity of samples. Short-length oligonucleotide DNA probes complementary to 16S or 23S rRNA sequences are generally used to target different phylogenetic levels. The protocol for the application of FISH to aggregated or suspended cells in mixed microbial communities is described in this chapter, with a special emphasis on environmental samples.

Keywords: Adherent cells; Environmental samples; FISH; Oligonucleotide probes; Suspension cells; Unculturable bacteria.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

In Situ Hybridization, Fluorescence / methods*
Microbiota / genetics
Oligonucleotide Probes / genetics
Phylogeny
RNA, Ribosomal, 16S / genetics
RNA, Ribosomal, 23S / genetics

Substances

Oligonucleotide Probes
RNA, Ribosomal, 16S
RNA, Ribosomal, 23S