We report the first experimental study of the intrinsic chemistry of a U-methylidyne species, focusing on reaction of [OUCH]+ with H2O, O2 and CH3C[triple bond, length as m-dash]N in the gas phase. DFT was also used to determine reaction pathways, and establish the mechanism by which [OUCH]+ is formed through collision-induced dissociation of [UO2(C[triple bond, length as m-dash]CH)]+.