Plastome of Saraca asoca (Detarioideae, Fabaceae): Annotation, comparison among subfamily and molecular typing

Saudi J Biol Sci. 2021 Feb;28(2):1487-1493. doi: 10.1016/j.sjbs.2020.12.008. Epub 2020 Dec 11.

Abstract

Saraca asoca (Roxb.) Willd. (subfamily Detarioideae, family Fabaceae) is a perennial evergreen sacred medicinal tree classified under 'vulnerable' by the IUCN. The chloroplast (cp) genome/plastome which follows uniparental inheritance contains many useful genetic information because of its conservative rate of evolution. The assembled cp genome of S. asoca which maps as a conserved circular structure revealed extensive rearrangement in gene organization, comprising total length 160,003 bp including LSC, SSC, IRa, and IRb, and GC content was 35.26%. Herein a set of rbcL and matK gene were established using molecular phylogenetic analyses for molecular typing of S. asoca.

Keywords: Detarioideae; Fabaceae; Genomic rearrangement; Molecular authentication; Plastome; Saraca asoca; Simple sequence repeat.