Abstract
Arachidonic acid is oxidized by the microsomal cytochrome P-450 monooxygenases of rabbit kidney cortex and medulla to products that include W and W-1 alcohols and several epoxyacids. In vivo administration of deoxycorticosterone acetate results in a time dependent and selective induction of the arachidonic acid W/W-1 oxygenase activity of cortex and medulla microsomal fractions (145% and 220% of controls, respectively). Initial segmental analysis shows the presence of an active epoxygenase activity in the isolated rabbit pars recta and its in vitro activation by p-aminohippuric acid.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Arachidonic Acids / metabolism*
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Chromatography, High Pressure Liquid
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Cytochrome P-450 CYP2J2
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Cytochrome P-450 Enzyme System / metabolism*
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Desoxycorticosterone / pharmacology
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Female
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Gas Chromatography-Mass Spectrometry
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Kidney / drug effects
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Kidney / enzymology*
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Kidney Cortex / drug effects
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Kidney Cortex / enzymology
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Kidney Medulla / drug effects
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Kidney Medulla / enzymology
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Microsomes / enzymology*
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Nephrons / enzymology
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Oxidation-Reduction
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Oxygenases / metabolism*
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Rabbits
Substances
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Arachidonic Acids
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Desoxycorticosterone
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Cytochrome P-450 Enzyme System
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Oxygenases
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Cytochrome P-450 CYP2J2