Protocol for human placental mesenchymal stem cell therapy in a murine model of intra-abdominal infection of hypervirulent Klebsiella

STAR Protoc. 2021 Feb 5;2(1):100337. doi: 10.1016/j.xpro.2021.100337. eCollection 2021 Mar 19.

Abstract

Hypervirulent Klebsiella pneumoniae (hvKP) strains cause extra-pulmonary infections such as intra-abdominal infection (IAI) even in healthy individuals due to its resistance to polymorphonuclear neutrophil (PMN) killing and a high incidence of multidrug resistance. To assess whether human placental mesenchymal stem cell (PMSC) therapy can be an effective treatment option, we established a murine model of hvKP-IAI to evaluate immune cell modulation and bacterial clearance for this highly lethal infection. This protocol can rapidly assess potential therapies for severe bacterial IAIs. For complete details on the use and execution of this protocol, please refer to Wang et al. (2020).

Keywords: Flow cytometry/mass cytometry; Immunology; Model organisms; Stem cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Female
  • Heterografts
  • Humans
  • Klebsiella Infections* / immunology
  • Klebsiella Infections* / therapy
  • Klebsiella pneumoniae / immunology*
  • Mesenchymal Stem Cell Transplantation*
  • Mesenchymal Stem Cells / immunology*
  • Mice
  • Placenta
  • Pregnancy