Karyopherin-β2 Inhibits and Reverses Aggregation and Liquid-liquid Phase Separation of the ALS/FTD-Associated Protein FUS

Emma Robinson; James Shorter; Lin Guo

doi:10.21769/BioProtoc.3725

Karyopherin-β2 Inhibits and Reverses Aggregation and Liquid-liquid Phase Separation of the ALS/FTD-Associated Protein FUS

Bio Protoc. 2020 Aug 20;10(16):e3725. doi: 10.21769/BioProtoc.3725.

Authors

Emma Robinson¹, James Shorter², Lin Guo¹

Affiliations

¹ Department of Biochemistry and Molecular Biology, Jefferson University, Philadelphia, PA 19107, USA.
² Department of Biochemistry and Biophysics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.

Abstract

The study of RNA-binding proteins (RBP) offers insight into the mechanisms of pathologic protein aggregation in neurodegenerative diseases. We developed a protocol for purifying an RBP FUS and a nuclear import receptor (NIR) Kapβ2 and testing the ability of Kapβ2 to mitigate FUS aggregation and liquid-liquid phase separation.

Keywords: FUS; Kapβ2; Liquid-liquid phase separation; Nuclear import receptor; Protein aggregation.

Grants and funding

R21 NS090205/NS/NINDS NIH HHS/United States