BRAF V600E mutation as a novel mechanism of acquired resistance to ALK inhibition in ALK-rearranged lung adenocarcinoma: A case report

Medicine (Baltimore). 2021 Feb 26;100(8):e24917. doi: 10.1097/MD.0000000000024917.

Abstract

Rationale: Patients with lung adenocarcinoma harboring EML4-ALK rearrangements respond well to multiple ALK tyrosine kinase inhibitors (TKIs). However, the tumor will invariably progress due to acquired resistance. Comprehensive genomic profiling appears to be a promising strategy to reveal the underlying molecular mechanisms of ALK-TKIs resistance.

Patient concerns: A patient with right lung adenocarcinoma harboring an ALK rearrangement received targeted therapy with multiple ALK-TKIs. He sought for follow-up treatment after his disease progressed again.

Diagnosis: The patient had a tumor diagnosed with stage I (T1bN0M0) lung adenocarcinoma.

Interventions: Due to the surgical contraindication, the patient did not undergo surgical resection. Instead, he received crizotinib as the first-line therapy with the progression-free survival of 20 months. Then he switched to alectinib treatment, however the disease rapidly progressed again.

Outcomes: Next-generation sequencing was performed and revealed that 7 somatic mutations were identified. Among them, 2 mutations, ALK I1171T and BRAF V600E, may be responsible for the resistance of this patient to ALK-TKIs. BRAF V600E mutation may explain the patient's resistance to lorlatinib.

Lessons: We present a case of ALK-rearranged lung adenocarcinoma with acquired resistance to ALK inhibition, in which the BRAF V600E mutation is a novel resistance mechanism. This provides evidence that BRAF V600E mutation is one mechanism of ALK-TKI resistance.

Publication types

  • Case Reports

MeSH terms

  • Adenocarcinoma of Lung / drug therapy
  • Adenocarcinoma of Lung / genetics*
  • Anaplastic Lymphoma Kinase / drug effects
  • Bone Neoplasms / drug therapy
  • Bone Neoplasms / genetics
  • Bone Neoplasms / secondary*
  • Cell Cycle Proteins
  • Crizotinib / pharmacology
  • Crizotinib / therapeutic use
  • Drug Resistance, Neoplasm / drug effects
  • Fatal Outcome
  • Humans
  • Lung Neoplasms / drug therapy
  • Lung Neoplasms / genetics*
  • Male
  • Microtubule-Associated Proteins
  • Middle Aged
  • Mutation
  • Protein Kinase Inhibitors / pharmacology
  • Protein Kinase Inhibitors / therapeutic use
  • Serine Endopeptidases

Substances

  • Cell Cycle Proteins
  • Microtubule-Associated Proteins
  • Protein Kinase Inhibitors
  • Crizotinib
  • Anaplastic Lymphoma Kinase
  • EML4 protein, human
  • Serine Endopeptidases