Discovery of a Potent and Orally Bioavailable Melatonin Receptor Agonist

J Med Chem. 2021 Mar 25;64(6):3059-3074. doi: 10.1021/acs.jmedchem.0c01836. Epub 2021 Mar 7.

Abstract

To develop potent and orally bioavailable melatonin receptor (MT1 and MT2) agonists, a novel series of 5-6-5 tricyclic derivatives was designed, synthesized, and evaluated. The synthesized indeno[5,4-d][1,3]oxazole, cyclopenta[c]pyrazolo[1,5-a]pyridine, indeno[5,4-d][1,3]thiazole, and cyclopenta[e]indazole derivatives showed potent binding affinities for MT1/MT2 receptors. Further optimization of these derivatives based on their metabolic stability in human hepatic microsomes revealed that (S)-3b ((S)-N-[2-(2-methyl-7,8-dihydro-6H-indeno[5,4-d][1,3]oxazol-8-yl)ethyl]acetamide) was a potent MT1 and MT2 ligand (MT1, Ki = 0.031 nM; MT2, Ki = 0.070 nM) with good metabolic stability in human hepatic microsomes. Moreover, compound (S)-3b showed good BBB permeability in rats, and its in vivo pharmacological effects were confirmed by its sleep-promotion ability in cats.

MeSH terms

  • Animals
  • Blood-Brain Barrier / metabolism
  • CHO Cells
  • Cricetulus
  • Drug Discovery
  • Humans
  • Indazoles / chemistry
  • Indazoles / pharmacokinetics
  • Indazoles / pharmacology*
  • Male
  • Pyridines / chemistry
  • Pyridines / pharmacokinetics
  • Pyridines / pharmacology*
  • Rats
  • Rats, Sprague-Dawley
  • Receptors, Melatonin / agonists*
  • Receptors, Melatonin / metabolism
  • Thiazoles / chemistry
  • Thiazoles / pharmacokinetics
  • Thiazoles / pharmacology*

Substances

  • Indazoles
  • Pyridines
  • Receptors, Melatonin
  • Thiazoles