Molecular epidemiology of Mycobacterium tuberculosis in Brazil before the whole genome sequencing era: a literature review

Mem Inst Oswaldo Cruz. 2021 Mar 15:116:e200517. doi: 10.1590/0074-02760200517. eCollection 2021.

Abstract

Molecular-typing can help in unraveling epidemiological scenarios and improvement for disease control strategies. A literature review of Mycobacterium tuberculosis transmission in Brazil through genotyping on 56 studies published from 1996-2019 was performed. The clustering rate for mycobacterial interspersed repetitive units - variable tandem repeats (MIRU-VNTR) of 1,613 isolates were: 73%, 33% and 28% based on 12, 15 and 24-loci, respectively; while for RFLP-IS6110 were: 84% among prison population in Rio de Janeiro, 69% among multidrug-resistant isolates in Rio Grande do Sul, and 56.2% in general population in São Paulo. These findings could improve tuberculosis (TB) surveillance and set up a solid basis to build a database of Mycobacterium genomes.

Publication types

  • Review

MeSH terms

  • Bacterial Typing Techniques
  • Brazil / epidemiology
  • Genotype
  • Humans
  • Minisatellite Repeats / genetics*
  • Molecular Epidemiology
  • Mycobacterium tuberculosis / genetics*
  • Mycobacterium tuberculosis / isolation & purification
  • Polymorphism, Restriction Fragment Length / genetics*
  • Whole Genome Sequencing