Non-modified RNA-Based Reprogramming of Human Dermal Fibroblasts into Induced Pluripotent Stem Cells

Immacolata Belviso; Franca Di Meglio; Veronica Romano; Stefania Montagnani; Clotilde Castaldo

doi:10.1007/7651_2021_364

Non-modified RNA-Based Reprogramming of Human Dermal Fibroblasts into Induced Pluripotent Stem Cells

Methods Mol Biol. 2022:2454:675-684. doi: 10.1007/7651_2021_364.

Authors

Immacolata Belviso¹, Franca Di Meglio¹, Veronica Romano¹, Stefania Montagnani¹, Clotilde Castaldo²

Affiliations

¹ Department of Public Health, University of Naples Federico II, Naples, Italy.
² Department of Public Health, University of Naples Federico II, Naples, Italy. [email protected].

PMID: 33730354
DOI: 10.1007/7651_2021_364

Abstract

The generation of pluripotent stem cells from adult somatic cells by cell reprogramming has put a whole new perspective on stem cell biology and stem cell-based regenerative medicine. Cell reprogramming acts through the introduction of key genes that regulate and maintain the pluripotent cell state. In this chapter, we describe the optimized protocol for the efficient isolation of fibroblasts from a skin punch biopsy and the subsequent easy and effective generation of integration-free induced pluripotent stem cell (iPSC) colonies forcing the expression of specific factors by non-modified RNAs.

Keywords: Cell Reprogramming; Dermal Fibroblasts; Induced Pluripotent Stem Cells (iPSCs); Integration-free; Non-modified RNAs (NM-RNAs); Regenerative Medicine.

MeSH terms

Adult
Cell Differentiation / genetics
Cellular Reprogramming / genetics
Fibroblasts / metabolism
Humans
Induced Pluripotent Stem Cells* / metabolism
Pluripotent Stem Cells* / metabolism
RNA / metabolism

Substances

RNA