A novel A > G polymorphism in the intron 2 of TBX3 gene is significantly associated with body size in donkeys

Gang Wang; Mei Li; Jun Zhou; Xiaoya An; Fuxia Bai; Yuan Gao; Jie Yu; Haijing Li; Chuzhao Lei; Ruihua Dang

doi:10.1016/j.gene.2021.145602

A novel A > G polymorphism in the intron 2 of TBX3 gene is significantly associated with body size in donkeys

Gene. 2021 Jun 15:785:145602. doi: 10.1016/j.gene.2021.145602. Epub 2021 Mar 23.

Authors

Gang Wang¹, Mei Li², Jun Zhou³, Xiaoya An⁴, Fuxia Bai⁵, Yuan Gao⁶, Jie Yu⁷, Haijing Li⁸, Chuzhao Lei⁴, Ruihua Dang⁹

Affiliations

¹ Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, Xianyang 712100, Shaanxi Province, China. Electronic address: [email protected].
² Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, Xianyang 712100, Shaanxi Province, China. Electronic address: [email protected].
³ Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, Xianyang 712100, Shaanxi Province, China. Electronic address: [email protected].
⁴ Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, Xianyang 712100, Shaanxi Province, China. Electronic address: [email protected].
⁵ Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, Xianyang 712100, Shaanxi Province, China. Electronic address: [email protected].
⁶ Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, Xianyang 712100, Shaanxi Province, China. Electronic address: [email protected].
⁷ Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, Xianyang 712100, Shaanxi Province, China; National Engineering Research Center for Gelatin-based Traditional Chinese Medicine, Dong-E-E-Jiao Co. Ltd., No. 78, E-jiao Street, Done-E Country, Shandong Province 252201, China. Electronic address: [email protected].
⁸ National Engineering Research Center for Gelatin-based Traditional Chinese Medicine, Dong-E-E-Jiao Co. Ltd., No. 78, E-jiao Street, Done-E Country, Shandong Province 252201, China. Electronic address: [email protected].
⁹ Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, Xianyang 712100, Shaanxi Province, China. Electronic address: [email protected].

PMID: 33766712
DOI: 10.1016/j.gene.2021.145602

Abstract

T-box transcription factor 3 (TBX3) gene encodes a transcriptional suppressor and plays an important role in embryonic development, which belongs to the T-box family. TBX3 also has been found to be associated with body size traits in horse that is a relative of donkey. Therefore, TBX3 is considered as a promising candidate gene for economic traits of donkey. This study aimed to reveal the significant variation of TBX3 gene in Dezhou donkey and explores the relationship between genotypes and body sizes. In this study, an A > G mutation was found in the intron 2 of TBX3 gene by sequencing, and three genotypes (AA, GG and AG) were identified in 380 Dezhou donkey individuals with Tm-shift method. Association analysis illustrated that there were significant differences between AA and GG genotype in body length, body height, chest depth, chest circumference, body weight, hucklebone width and rump length. Our results demonstrated that the polymorphism of TBX3 is significantly associated with body size traits, which can serve as a marker to improve donkey production performance.

Keywords: Body size trait; Donkey; SNP; TBX3 gene; Tm-shift.

MeSH terms

Animals
Body Size / genetics*
Equidae / anatomy & histology*
Equidae / genetics*
Female
Genotype
Introns / genetics
Male
Mutation
Polymorphism, Single Nucleotide
T-Box Domain Proteins / genetics*
T-Box Domain Proteins / physiology

Substances

T-Box Domain Proteins