The TAZ domain-containing proteins play important role in the heavy metals stress biology in plants

Abdullah Shalmani; Uzair Ullah; Izhar Muhammad; Dong Zhang; Rahat Sharif; Peng Jia; Noor Saleem; Nazish Gul; Aizhan Rakhmanova; Muhammad Mobeen Tahir; Kun-Ming Chen; Na An

doi:10.1016/j.envres.2021.111030

The TAZ domain-containing proteins play important role in the heavy metals stress biology in plants

Environ Res. 2021 Jun:197:111030. doi: 10.1016/j.envres.2021.111030. Epub 2021 Mar 24.

Authors

Abdullah Shalmani¹, Uzair Ullah², Izhar Muhammad³, Dong Zhang⁴, Rahat Sharif⁵, Peng Jia⁶, Noor Saleem⁷, Nazish Gul⁸, Aizhan Rakhmanova⁹, Muhammad Mobeen Tahir¹⁰, Kun-Ming Chen¹¹, Na An¹²

Affiliations

¹ College of Horticulture, Northwest A and F University, Yangling, Shaanxi Province, 712100, China; State Key Laboratory of Crop Stress Biology in Arid Areas, College of Life Sciences, Northwest A&F University, Yangling, Shaanxi Province, 712100, China. Electronic address: [email protected].
² Department of Genetics, Hazara University, Manshera, KPK, Pakistan. Electronic address: [email protected].
³ State Key Laboratory of Crop Stress Biology in Arid Areas, College of Life Sciences, Northwest A&F University, Yangling, Shaanxi Province, 712100, China; College of Agronomy, Northwest A and F University, Yangling, Shaanxi Province, 712100, China. Electronic address: [email protected].
⁴ College of Horticulture, Northwest A and F University, Yangling, Shaanxi Province, 712100, China. Electronic address: [email protected].
⁵ Department of Horticulture, School of Horticulture and Plant Protection, Yangzhou University, 48 Wenhui East Road, Yangzhou, Jiangsu, 225009, PR China. Electronic address: [email protected].
⁶ College of Horticulture, Northwest A and F University, Yangling, Shaanxi Province, 712100, China. Electronic address: [email protected].
⁷ College of Agronomy, Northwest A and F University, Yangling, Shaanxi Province, 712100, China. Electronic address: [email protected].
⁸ Department of Genetics, Hazara University, Manshera, KPK, Pakistan. Electronic address: [email protected].
⁹ College of Food Science and Engineering, Northwest A & F University, Yangling, Shaanxi Province, 712100, China. Electronic address: [email protected].
¹⁰ College of Horticulture, Northwest A and F University, Yangling, Shaanxi Province, 712100, China. Electronic address: [email protected].
¹¹ State Key Laboratory of Crop Stress Biology in Arid Areas, College of Life Sciences, Northwest A&F University, Yangling, Shaanxi Province, 712100, China. Electronic address: [email protected].
¹² College of Horticulture, Northwest A and F University, Yangling, Shaanxi Province, 712100, China; College of Life Sciences, Northwest A&F University, Yangling, Shaanxi Province, 712100, China. Electronic address: [email protected].

PMID: 33774015
DOI: 10.1016/j.envres.2021.111030

Abstract

TAZ (transcriptional coactivator with PDZ-binding) zinc finger domains, also known as transcription adaptor putative zinc finger domains, that control diverse function in plant growth and development. Here, in the present study, we evaluated the role of the TAZ domain-containing gene in response to various heavy metals. Initially, we found a total of 3, 7, 8, 9, 9, 9, 7, 14, 6, 10, and 6 proteins containing TAZ domain in stiff brome, millet, sorghum, potato, pepper, maize, rice, apple, peach, pear, and tomato genome that could trigger the plant resistance against various heavy metals, respectively. Various in-silico approaches were applied such as duplication, phylogenetic analysis, and gene structure, to understand the basic features of the TAZ domain-containing genes in plants. Gene expression analyses were also performed under heavy metals (Cr, Zn, Ni, Cd, Co, Fe, Mn, and Pb). The results of quantitative real-time PCR analysis indicated that the TAZ gene family members were differentially expressed under different heavy metals. We further characterized the functions of the TAZ domain-containing gene under the heavy metal stresses by overexpressing the OsTAZ4 gene in Arabidopsis. The TAZ genes could promote plant resistance against various heavy metals by interacting with OsMYB34 and OsFHA9 transcription factors. The results will contribute to elucidate the relationship of TAZ proteins with heavy metals stresses and also ascertain the biological function in plant growth and development.

Keywords: Apple; Domain; Proteins; Rice; TAZ.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biology
Metals, Heavy* / toxicity
Oryza*
Phylogeny
Transcription Factors / genetics

Substances

Metals, Heavy
Transcription Factors