3-Azi-1-methoxybutyl beta-D-galactopyranoside, a photoaffinity label for monoclonal antigalactan antibodies of the VH GAL 39.1/55.1 gene-family

G L Jung; K R Holme; C P Glaudemans; J Lehmann; H Lay

doi:10.1016/0008-6215(88)85083-3

3-Azi-1-methoxybutyl beta-D-galactopyranoside, a photoaffinity label for monoclonal antigalactan antibodies of the VH GAL 39.1/55.1 gene-family

Carbohydr Res. 1988 Mar 15:174:87-98. doi: 10.1016/0008-6215(88)85083-3.

Authors

G L Jung¹, K R Holme, C P Glaudemans, J Lehmann, H Lay

Affiliation

¹ National Institutes of Health, Bethesda, MD 20892.

PMID: 3378234
DOI: 10.1016/0008-6215(88)85083-3

Abstract

3-Azi-1-methoxybutyl beta-D-galactopyranoside, prepared from 2,3,4,6-tetra-O-acetyl-alpha-D-galactopyranosyl bromide in four steps, had an affinity constant for antigalactans IgA (Fab') J539 and X24 of 1550 and 1730 M-1, respectively. 3-Azi-1-methoxybutyl beta-D-(4-3H)galactopyranoside, when photolysed in the presence of IgA X24, specifically labelled the galactan-binding area of the immunoglobulin.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Affinity Labels / chemical synthesis*
Antibodies, Monoclonal*
Galactans / genetics*
Galactans / immunology
Galactosides / chemical synthesis*
Glycosides / chemical synthesis*
Immunoglobulin A
Ligands
Magnetic Resonance Spectroscopy
Optical Rotation
Spectrometry, Fluorescence

Substances

Affinity Labels
Antibodies, Monoclonal
Galactans
Galactosides
Glycosides
Immunoglobulin A
Ligands
3-azi-1-methoxybutylgalactopyranoside