Genome-scale metabolic network model (GSMM) is an extremely important guiding tool in the targeted modification of industrial microbial strains, which helps researchers to quickly obtain industrial microbes with specific traits and has attracted increasing attention. Here we reviewe the development history of GSMM and summarized the construction method of GSMM. Furthermore, the development and application of GSMM in industrial microorganisms are elaborated by using four typical industrial microorganisms (Bacillus subtilis, Escherichia coli, Corynebacterium glutamicum, and Saccharomyces cerevisiae) as examples. In addition, prospects in the development trend of GSMM are proposed.
基因组规模代谢网络 (Genome-scale metabolic network model，GSMM) 是工业微生物菌株定向改造过程中一种极为重要的指导性工具，有助于研究者快速获取特定性状的工业微生物，因此越来越受到人们的关注。文中回顾了GSMM的发展历程，总结并评述了GSMM的构建方法，以4种重要工业微生物 (枯草芽孢杆菌Bacillus subtilis、大肠杆菌Escherichia coli、谷氨酸棒杆菌Corynebacterium glutamicum和酿酒酵母Saccharomyces cerevisiae)为例，阐述了GSMM在工业微生物中的发展与应用。此外，还对GSMM未来的发展趋势进行了展望。.
Keywords: genome-scale metabolic network model; model application; model construction; model evaluate indicators; typical industrial microorganisms.