Multi-walled carbon nanotube polysaccharide modified Hericium erinaceus polysaccharide as an adjuvant to extend immune responses

Int J Biol Macromol. 2021 Jul 1:182:574-582. doi: 10.1016/j.ijbiomac.2021.03.180. Epub 2021 Mar 30.

Abstract

In recent years, the utilization of CS-MWCNT as targeted drug carriers has attracted considerable attention. Hericium erinaceus polysaccharide (HEP) has been reported as an immunostimulant to improve immune responses. This study was focussed on developing CS-MWCNT encapsulating HEP (CS-MWCNT-HEP). Using in mice peritoneal macrophages, we found the immune response could be effectively regulated by CS-MWCNT-HEP, promoted the expression of the MHCII, CD86, F4/80 and gp38. Moreover, the mice immunized with CS-MWCNT-HEP nanoparticles significantly extended PCV2-specific IgG immune response and the levels of cytokines. The results demonstrated that CS-MWCNT-HEP may be a promising drug delivery system for immuno-enhancement.

Keywords: Adjuvant; Hericium erinaceus polysaccharide; Immune responses; Multi-walled carbon nanotube (MWCNT); PCV(2).

MeSH terms

  • Adjuvants, Immunologic / chemical synthesis*
  • Adjuvants, Immunologic / pharmacology
  • Animals
  • Antibodies, Viral / immunology
  • Cells, Cultured
  • Circovirus / immunology
  • Cytokines / immunology
  • Fungal Polysaccharides / chemistry*
  • Fungal Polysaccharides / immunology
  • Hericium / chemistry
  • Immunogenicity, Vaccine
  • Macrophages, Peritoneal / drug effects
  • Macrophages, Peritoneal / immunology
  • Mice
  • Mice, Inbred ICR
  • Nanotubes, Carbon / chemistry*

Substances

  • Adjuvants, Immunologic
  • Antibodies, Viral
  • Cytokines
  • Fungal Polysaccharides
  • Nanotubes, Carbon

Supplementary concepts

  • Hericium erinaceus