Objective: To investigate the effect of long non-coding RNA-TUC338 on the proliferation and migration of lymphoma cells.
Methods: The expression of TUC338 in different lymphoma cells was detected by fluorescence quantitative PCR, cell proliferation by sulforhodamine B (SRB) assay, migration of lymphoma cells by transwell assay, and protein expression in PI3K/AKT signaling pathway by Western blot.
Results: The expression levels of TUC338 in lymphoma cells Daudi, U937, BC-3, and Raji significantly increased in comparison with human normal T lymphocytes H9 (t=13.277, 10.103, 16.200, and 26.687, P=0.002, 0.005, 0.001, and 0.000). Compared with NC-siRNA group, the number of cells crossing the chamber of TUC338-siRNA group was significantly reduced (t=30.508, P=0.000), the protein expression levels of p-PI3K and p-AKT significantly decreased (t=16.872 and 18.371, P=0.000 and 0.000), and OD530 absorbance values at 24 h, 48 h, and 72 h were significantly lower (P<0.05).
Conclusion: The expression of TUC338 significantly increases in lymphoma cells, and silence of TUC338 effectively inhibits the activation of PI3K/AKT signaling pathway, thereby inhibiting the proliferation and migration of lymphoma cells, which has a potential application value in diagnosis and treatment of lymphoma.
题目: 长链非编码RNA-TUC338通过PI3K/AKT信号通路对淋巴瘤细胞迁移和增殖的影响.
目的: 探讨长链非编码RNA-TUC338对淋巴瘤细胞增殖和迁移的影响.
方法: 采用荧光定量PCR检测不同淋巴瘤细胞中TUC338表达，磺酰罗丹明B实验检测细胞增殖能力，细胞迁移侵袭实验检测淋巴瘤细胞迁移能力，蛋白免疫印迹实验检测PI3K/AKT信号通路蛋白表达情况.
结果: 与人正常T淋巴细胞H9相比，淋巴瘤细胞Daudi、U937、BC-3和Raji中TUC338表达水平均明显提高（t=13.277、10.103、16.200和26.687，P=0.002、0.005、0.001和0.000）。与NC-siRNA组相比，TUC338-siRNA组穿过小室细胞数明显减少（t=30.508，P=0.000），p-PI3K和p-AKT蛋白表达量明显下降（t=16.872、18.371，P=0.000、0.000），在24、48和72 h的OD530吸光值均明显偏低（P<0.05）.
结论: 在淋巴瘤细胞中TUC338表达量明显增加，沉默TUC338表达能够有效抑制PI3K/AKT信号通路的激活，进而抑制淋巴瘤细胞的增殖和迁移，在淋巴瘤诊断和治疗中具有潜在应用价值.