Analysis of Saccharification Products of High-Concentration Glutinous Rice Fermentation by Rhizopus nigricans Q3 and Alcoholic Fermentation of Saccharomyces cerevisiae GY-1

Junjun Li; Xianghua Tang; Hong Qian; Yunjuan Yang; Xuan Zhu; Qian Wu; Yuelin Mu; Zunxi Huang

doi:10.1021/acsomega.0c05452

Analysis of Saccharification Products of High-Concentration Glutinous Rice Fermentation by Rhizopus nigricans Q3 and Alcoholic Fermentation of Saccharomyces cerevisiae GY-1

ACS Omega. 2021 Mar 18;6(12):8038-8044. doi: 10.1021/acsomega.0c05452. eCollection 2021 Mar 30.

Authors

Junjun Li^{1

2}, Xianghua Tang^{1

2}, Hong Qian², Yunjuan Yang^{1

2}, Xuan Zhu³, Qian Wu^{1

2

4}, Yuelin Mu^{1

2

4}, Zunxi Huang^{1

2

4}

Affiliations

¹ Engineering Research Center of Sustainable Development and Utilization of Biomass Energy, Ministry of Education and School of Life Science, Yunnan Normal University, Kunming 650500, P. R. China.
² School of Life Science, Yunnan Normal University, Kunming 650500, P. R. China.
³ Guangdong Haid Group Co., Ltd., Guangzhou 51400, P. R. China.
⁴ Key Laboratory of Yunnan for Biomass Energy and Biotechnology of Environment, Kunming 650500, P. R. China.

Abstract

A two-stage process was used to prepare rice alcohol, i.e., saccharification of glutinous rice by Rhizopus nigricans Q3, followed by Saccharomyces cerevisiae's fermentation. Rhizopus nigricans Q3 was cultured during the saccharification stage, and Saccharomyces cerevisiae GY-1 was added in the fermentation stage. Total sugar content and reducing sugar content in these two stages were analyzed. The relationship between the production proportion and consumption of the reducing sugar in the saccharification interval was analyzed using reducing sugar indices. It is an important rule that the high-concentration syrup and oligosaccharides prepared by glutinous rice could reach 42°Bx and 250 mg/mL by high-concentration fermentation in the growth stage of R. nigricans Q3.