Ultra-sensitive Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Antigen Detection for the Diagnosis of Coronavirus Disease 2019 (COVID-19) in Upper Respiratory Samples

Hannah Wang; Catherine A Hogan; Michelle Verghese; Daniel Solis; Mamdouh Sibai; ChunHong Huang; James Zehnder; Malaya K Sahoo; Benjamin A Pinsky

doi:10.1093/cid/ciab063

Ultra-sensitive Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Antigen Detection for the Diagnosis of Coronavirus Disease 2019 (COVID-19) in Upper Respiratory Samples

Clin Infect Dis. 2021 Dec 16;73(12):2326-2328. doi: 10.1093/cid/ciab063.

Authors

Hannah Wang¹, Catherine A Hogan¹, Michelle Verghese¹, Daniel Solis¹, Mamdouh Sibai¹, ChunHong Huang¹, James Zehnder¹, Malaya K Sahoo¹, Benjamin A Pinsky^{1

2}

Affiliations

¹ Department of Pathology, Stanford University School of Medicine, Stanford, California, USA.
² Division of Infectious Diseases and Geographic Medicine, Department of Medicine, Stanford University School of Medicine, Stanford, California, USA.

Abstract

An ultra-sensitive severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) nucleocapsid antigen assay (S-PLEX, MesoScale Diagnostics) was evaluated in 250 retrospective and 200 prospective upper respiratory specimens. In samples with cycle threshold <35, there was 95%-98% positive and 93%-96% negative percent agreement with reverse transcription-polymerase chain reaction. S-PLEX may provide a high-throughput alternative to nucleic acid-based testing for coronavirus disease 2019 (COVID-19) diagnosis.

Keywords: COVID-19; COVID-19 testing; SARS-COV-2 antigen testing; SARS-CoV-2.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

COVID-19*
Humans
Immunologic Tests
Prospective Studies
Retrospective Studies
SARS-CoV-2*

Grants and funding

MesoScale Diagnostics