Differential Expression of Potential Biomarkers of Oral Squamous Cell Carcinoma Development

Head Neck Pathol. 2021 Dec;15(4):1127-1136. doi: 10.1007/s12105-021-01322-8. Epub 2021 Apr 11.

Abstract

To evaluate molecular epithelial changes, we investigated whether a profile of survivin, cyclin dependent kinase inhibitor 2A (CDKN2A), epidermal growth factor receptor (EGFR), polo like kinase 1 (PLK1), p63, p40 (Δnp63 isoform), cyclin D1 (CCND1) and BCL2 apoptosis regulator (BCL2) proteins could predict malignant transformation. Different tissue segments (tumor adjacent epithelium; dysplasia and tumor) from a total of 109 patients were analyzed by immunohistochemistry. An increased expression of survivin (p < 0.001), PLK1 (p = 0.001), and p63 (p < 0.001) in parallel to reduced immunostaining of p40 (p < 0.001) and BCL2 (p = 0.029) was observed among the tissue segments analyzed. Our study revealed that survivin, PLK1, p63, p40 and BCL2 play a role in oral tumorigenesis and represent promising biomarkers able to recognize mesenchymal phenotype induction in the transition from nonmalignant cells to tumor cells. These results reveals critical interaction between survivin, PLK1, p63, p40 promising proteins during invasive carcinoma development.

Keywords: Biomarkers; Dysplasia; Immunohistochemistry; Oral cancer; Progression; Tumor.

Publication types

  • Multicenter Study

MeSH terms

  • Biomarkers, Tumor / metabolism
  • Carcinoma, Squamous Cell / metabolism*
  • Carcinoma, Squamous Cell / pathology
  • Cell Cycle Proteins / metabolism
  • Cell Transformation, Neoplastic / pathology
  • Cyclin D1 / metabolism
  • Cyclin-Dependent Kinase Inhibitor p16 / metabolism
  • ErbB Receptors / metabolism
  • Female
  • Humans
  • Immunohistochemistry
  • Male
  • Middle Aged
  • Mouth Mucosa / metabolism*
  • Mouth Neoplasms / metabolism*
  • Mouth Neoplasms / pathology
  • Polo-Like Kinase 1
  • Protein Isoforms
  • Protein Serine-Threonine Kinases / metabolism
  • Proto-Oncogene Proteins / metabolism
  • Proto-Oncogene Proteins c-bcl-2 / metabolism
  • Survivin / metabolism
  • Transcription Factors / metabolism

Substances

  • Biomarkers, Tumor
  • Cell Cycle Proteins
  • Cyclin-Dependent Kinase Inhibitor p16
  • Protein Isoforms
  • Proto-Oncogene Proteins
  • Proto-Oncogene Proteins c-bcl-2
  • Survivin
  • Transcription Factors
  • Cyclin D1
  • ErbB Receptors
  • Protein Serine-Threonine Kinases