Use of a solid phase red blood cell adherence method for pretransfusion platelet compatibility testing

Am J Clin Pathol. 1988 Jul;90(1):63-8. doi: 10.1093/ajcp/90.1.63.

Abstract

A solid phase red blood cell adherence method has been used for platelet antibody detection and crossmatching for refractory platelet recipients. Patient sera were first screened for HLA or platelet-specific antibodies, then crossmatched with potential apheresis platelet donors. The overall correlation of platelet crossmatch results with transfusion outcome was 97% in patients with no evidence of nonimmune platelet destruction. The solid phase red blood cell adherence method provided a feasible and effective alternative to HLA matching as a means of donor selection for refractory platelet recipients. The speed and simplicity of this method may allow most hospital laboratories to perform platelet antibody screening before routine platelet transfusions.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies / analysis
  • Blood Component Removal
  • Blood Grouping and Crossmatching*
  • Blood Platelets / immunology*
  • Blood Transfusion*
  • Cell Adhesion
  • Erythrocytes / physiology*
  • HLA Antigens / analysis
  • Humans
  • Platelet Transfusion
  • Prospective Studies

Substances

  • Antibodies
  • HLA Antigens