Biomimetic Ca2+ nanogenerator based on ions interference strategy for tumour-specific therapy

J Drug Target. 2021 Dec;29(10):1094-1101. doi: 10.1080/1061186X.2021.1919123. Epub 2021 May 3.

Abstract

Intracellular Ca2+ ions as second messenger played key role in cell behaviour, which was often overlooked in traditional antitumor treatment. Disrupting Ca2+ ion homeostasis by Ca2+ overload might switch ions signal from 'regulating' to 'destroying'. Inspired by this, a biomimetic Ca2+ nanogenerator was constructed. Briefly, the curcumin (CUR) was loaded into mesoporous calcium carbonate nanoparticles (MCC NPs), and then coated with platelet (PLT) membrane. Upon reaching tumour cells by PLT membrane-mediated tumour targeting effect, PLT@MCC/CUR would instantaneously decompose in acidic lysosomes, concurrently accompanying with Ca2+ generation and CUR release. The CUR could further facilitate Ca2+ release from endoplasmic reticulum (ER) and inhibit Ca2+ efflux, aggravating Ca2+ overload to disrupt mitochondrial Ca2+ homeostasis for mitochondria apoptosis signalling pathway activation. Interestingly, such effect was ineffective in normal cells, realising the tumour-specific therapeutic therapy. Based on ions interference strategy, PLT@MCC/CUR herein offered synergistic combination of Ca2+ overload therapy and chemotherapy, which would pave the way towards more effective nanotherapeutics.

Keywords: Ions interference; biomimetic; calcium carbonate; calcium overload; curcumin.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antineoplastic Agents / administration & dosage
  • Antineoplastic Agents / pharmacology
  • Apoptosis / drug effects
  • Biomimetics
  • Blood Platelets / chemistry
  • Breast Neoplasms / drug therapy*
  • Calcium / metabolism
  • Calcium Carbonate / chemistry*
  • Curcumin / administration & dosage
  • Curcumin / pharmacology*
  • Drug Liberation
  • Female
  • Homeostasis
  • Humans
  • MCF-7 Cells
  • Mice
  • Mice, Inbred BALB C
  • Mice, Nude
  • Mitochondria / drug effects
  • Nanoparticles*
  • RAW 264.7 Cells

Substances

  • Antineoplastic Agents
  • Calcium Carbonate
  • Curcumin
  • Calcium