DIA-MSE to Study Microglial Function in Schizophrenia

Guilherme Reis-de-Oliveira; Victor Corasolla Carregari; Daniel Martins-de-Souza

doi:10.1007/978-1-0716-1024-4_24

DIA-MS^E to Study Microglial Function in Schizophrenia

Methods Mol Biol. 2021:2228:341-352. doi: 10.1007/978-1-0716-1024-4_24.

Authors

Guilherme Reis-de-Oliveira¹, Victor Corasolla Carregari¹, Daniel Martins-de-Souza^{2

3

4

5}

Affiliations

¹ Lab of Neuroproteomics, Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, Brazil.
² Lab of Neuroproteomics, Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, Brazil. [email protected].
³ Instituto Nacional de Biomarcadores em Neuropsiquiatria (INBION), Conselho Nacional de Desenvolvimento Científico e Tecnológico, São Paulo, Brazil. [email protected].
⁴ Experimental Medicine Research Cluster (EMRC), University of Campinas, Campinas, Brazil. [email protected].
⁵ D'Or Institute for Research and Education (IDOR), São Paulo, Brazil. [email protected].

PMID: 33950502
DOI: 10.1007/978-1-0716-1024-4_24

Abstract

Here, we describe a proteomic pipeline to use a human microglial cell line as a biological model to study schizophrenia. In order to maximize the proteome coverage, we apply two-dimensional liquid chromatography coupled with ultra-definition MS^E mass spectrometry (LC-UDMS^E) using a data-independent acquisition (DIA) approach, with an optimization of drift time collision energy.

Keywords: DIA; MSE; Mass spectrometry; Microglia; Schizophrenia; UDMSE.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Line
Chromatography, Liquid
Humans
Microglia / metabolism*
Proteins / analysis*
Proteome*
Proteomics*
Research Design
Schizophrenia / metabolism*
Spectrometry, Mass, Electrospray Ionization*
Tandem Mass Spectrometry*

Substances

Proteins
Proteome