Prognostic value of members of NFAT family for pan-cancer and a prediction model based on NFAT2 in bladder cancer

Aging (Albany NY). 2021 May 7;13(10):13876-13897. doi: 10.18632/aging.202982. Epub 2021 May 7.

Abstract

Bladder cancer (BLCA) is one of the common malignant tumors of the urinary system. The poor prognosis of BLCA patients is due to the lack of early diagnosis and disease recurrence after treatment. Increasing evidence suggests that gene products of the nuclear factor of activated T-cells (NFAT) family are involved in BLCA progression and subsequent interaction(s) with immune surveillance. In this study, we carried out a pan-cancer analysis of the NFAT family and found that NFAT2 is an independent prognostic factor for BLCA. We then screened for differentially expressed genes (DEGs) and further analyzed such candidate gene loci using gene ontology enrichment to curate the KEGG database. We then used Lasso and multivariate Cox regression to identify 4 gene loci (FER1L4, RNF128, EPHB6, and FN1) which were screened together with NFAT2 to construct a prognostic model based on using Kaplan-Meier analysis to predict the overall survival of BLCA patients. Moreover, the accuracy of our proposed model is supported by deposited datasets in the Gene Expression Omnibus (GEO) database. Finally, a nomogram of this prognosis model for BLCA was established which could help to provide better disease management and treatment.

Keywords: NFAT; bladder cancer; nomogram; overall survival; prognostic risk score.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Line, Tumor
  • Gene Expression Profiling
  • Gene Expression Regulation, Neoplastic
  • Gene Ontology
  • Humans
  • Kaplan-Meier Estimate
  • Models, Biological*
  • NFATC Transcription Factors / genetics
  • NFATC Transcription Factors / metabolism*
  • Oncogenes
  • Prognosis
  • Proportional Hazards Models
  • Protein Interaction Maps / genetics
  • Reproducibility of Results
  • Risk Factors
  • Urinary Bladder Neoplasms / diagnosis*
  • Urinary Bladder Neoplasms / genetics
  • Urinary Bladder Neoplasms / metabolism*
  • Urinary Bladder Neoplasms / pathology

Substances

  • NFATC Transcription Factors