BENZ WS: the Bologna ENZyme Web Server for four-level EC number annotation

Davide Baldazzi; Castrense Savojardo; Pier Luigi Martelli; Rita Casadio

doi:10.1093/nar/gkab328

BENZ WS: the Bologna ENZyme Web Server for four-level EC number annotation

Nucleic Acids Res. 2021 Jul 2;49(W1):W60-W66. doi: 10.1093/nar/gkab328.

Authors

Davide Baldazzi¹, Castrense Savojardo¹, Pier Luigi Martelli¹, Rita Casadio^{1

2}

Affiliations

¹ Biocomputing Group, Department of Pharmacy and Biotechnologies, University of Bologna, Italy.
² Institute of Biomembranes, Bioenergetics and Molecular Biotechnologies (IBIOM), Italian National Research Council (CNR), Bari, Italy.

Abstract

The Bologna ENZyme Web Server (BENZ WS) annotates four-level Enzyme Commission numbers (EC numbers) as defined by the International Union of Biochemistry and Molecular Biology (IUBMB). BENZ WS filters a target sequence with a combined system of Hidden Markov Models, modelling protein sequences annotated with the same molecular function, and Pfams, carrying along conserved protein domains. BENZ returns, when successful, for any enzyme target sequence an associated four-level EC number. Our system can annotate both monofunctional and polyfunctional enzymes, and it can be a valuable resource for sequence functional annotation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Enzymes / chemistry*
Internet
Markov Chains
Molecular Sequence Annotation / methods*
Protein Domains
Sequence Alignment
Sequence Analysis, Protein / methods*
Software*

Substances

Enzymes