Time lapse recording of cortical interneuron migration in mouse organotypic brain slices and explants

Fanny Lepiemme; Carla G Silva; Laurent Nguyen

doi:10.1016/j.xpro.2021.100467

Time lapse recording of cortical interneuron migration in mouse organotypic brain slices and explants

STAR Protoc. 2021 Apr 16;2(2):100467. doi: 10.1016/j.xpro.2021.100467. eCollection 2021 Jun 18.

Authors

Fanny Lepiemme¹, Carla G Silva¹, Laurent Nguyen¹

Affiliation

¹ GIGA-Stem Cells and GIGA-Neurosciences, Interdisciplinary Cluster for Applied Genoproteomics (GIGA-R), University of Liège, CHU Sart-Tilman, Liège 4000, Belgium.

Abstract

Interneuron migration involves repetitive cycles of pausing and motion that include nucleokinesis and dynamic branching of the leading process. Here, we provide a step-by-step description of how to culture and record the migration of cortical interneurons. We provide two culture models: the first includes organotypic brain slices and the second medial ganglionic eminence (MGE) explants. While organotypic brain slices provide a close-to-physiological context to analyze interneuron migration into cortical streams, MGE explants are appropriate to investigate the fine details of interneuron morphology remodeling during movement. For complete details on the use and execution of this protocol, please refer to Silva et al. (2018).

Keywords: Cell Biology; Microbiology; Neuroscience.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Movement*
Cerebral Cortex* / cytology
Cerebral Cortex* / metabolism
Interneurons* / cytology
Interneurons* / metabolism
Mice
Microdissection*
Tissue Culture Techniques*