Epitope-targeting platform for broadly protective influenza vaccines

PLoS One. 2021 May 27;16(5):e0252170. doi: 10.1371/journal.pone.0252170. eCollection 2021.

Abstract

Seasonal influenza vaccines are often ineffective because they elicit strain-specific antibody responses to mutation-prone sites on the hemagglutinin (HA) head. Vaccines that provide long-lasting immunity to conserved epitopes are needed. Recently, we reported a nanoparticle-based vaccine platform produced by solid-phase peptide synthesis (SPPS) for targeting linear and helical protein-based epitopes. Here, we illustrate its potential for building broadly protective influenza vaccines. Targeting known epitopes in the HA stem, neuraminidase (NA) active site, and M2 ectodomain (M2e) conferred 50-75% survival against 5LD50 influenza B and H1N1 challenge; combining stem and M2e antigens increased survival to 90%. Additionally, protein sequence and structural information were employed in tandem to identify alternative epitopes that stimulate greater protection; we report three novel HA and NA sites that are highly conserved in type B viruses. One new target in the HA stem stimulated 100% survival, highlighting the value of this simple epitope discovery strategy. A candidate influenza B vaccine targeting two adjacent HA stem sites led to >104-fold reduction in pulmonary viral load. These studies describe a compelling platform for building vaccines that target conserved influenza epitopes.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibodies, Viral / immunology*
  • Epitopes / immunology*
  • Hemagglutinin Glycoproteins, Influenza Virus / immunology
  • Humans
  • Influenza B virus / immunology*
  • Influenza, Human* / immunology
  • Influenza, Human* / prevention & control
  • Influenza, Human* / virology
  • Mice
  • Orthomyxoviridae Infections* / immunology
  • Orthomyxoviridae Infections* / virology

Substances

  • Antibodies, Viral
  • Epitopes
  • Hemagglutinin Glycoproteins, Influenza Virus