Abstract
A hydrophilic probe is employed to enrich exosomes from three kinds of cancer cells by TiO2-phosphate interaction and exosomal glycoproteins by hydrophilic interaction in succession. The probe performs efficiently in both the enrichment processes. And the analytical results confirm that unique exosomal glycoproteins can distinguish parent exosomes from others.
MeSH terms
-
Cell Line, Tumor
-
Exosomes / metabolism*
-
Ferrosoferric Oxide / chemistry
-
Glutathione / chemistry
-
Glutathione / metabolism
-
Glycoproteins / analysis*
-
Glycoproteins / metabolism
-
Glycosylation
-
Humans
-
Hydrophobic and Hydrophilic Interactions
-
Magnetite Nanoparticles / chemistry
-
Molecular Probes / chemistry
-
Molecular Probes / metabolism*
-
Proteomics / methods
-
Titanium / chemistry
Substances
-
Glycoproteins
-
Magnetite Nanoparticles
-
Molecular Probes
-
titanium dioxide
-
Titanium
-
Glutathione
-
Ferrosoferric Oxide