Generation of three human iPSC lines from patients with a spontaneous late-onset Alzheimer's disease and three sex- and age-matched healthy controls

Jan Raska; Hana Klimova; Katerina Sheardova; Veronika Fedorova; Hana Hribkova; Veronika Pospisilova; Simona Vochyanova; Tereza Vanova; Dasa Bohaciakova

doi:10.1016/j.scr.2021.102378

Generation of three human iPSC lines from patients with a spontaneous late-onset Alzheimer's disease and three sex- and age-matched healthy controls

Stem Cell Res. 2021 May:53:102378. doi: 10.1016/j.scr.2021.102378. Epub 2021 Apr 29.

Authors

Jan Raska¹, Hana Klimova¹, Katerina Sheardova², Veronika Fedorova¹, Hana Hribkova¹, Veronika Pospisilova¹, Simona Vochyanova¹, Tereza Vanova³, Dasa Bohaciakova⁴

Affiliations

¹ Department of Histology and Embryology, Faculty of Medicine, Masaryk University, Brno, Czech Republic.
² International Clinical Research Center (ICRC) and Neurology Department, St. Anne's University Hospital, Brno, Czech Republic; Neurology Department, St. Anne's University Hospital, Brno, Czech Republic.
³ Department of Histology and Embryology, Faculty of Medicine, Masaryk University, Brno, Czech Republic; International Clinical Research Center (ICRC) and Neurology Department, St. Anne's University Hospital, Brno, Czech Republic.
⁴ Department of Histology and Embryology, Faculty of Medicine, Masaryk University, Brno, Czech Republic; International Clinical Research Center (ICRC) and Neurology Department, St. Anne's University Hospital, Brno, Czech Republic. Electronic address: [email protected].

PMID: 34088007
DOI: 10.1016/j.scr.2021.102378

Abstract

Human induced pluripotent stem cell (iPSC) lines were generated from patients with spontaneous late-onset Alzheimer's disease (AD) and three healthy control individuals. Peripheral blood mononuclear cells were reprogrammed with Yamanaka factors (OSKM) using a commercially available Epi5 Reprogramming Kit. The pluripotency of iPSCs was confirmed by the expression of pluripotency factors and by their ability to differentiate to all three germ layers in vitro. Newly derived cell lines can be used to model Alzheimer's disease in vitro.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Alzheimer Disease*
Cell Differentiation
Cellular Reprogramming
Germ Layers
Humans
Induced Pluripotent Stem Cells*
Leukocytes, Mononuclear