Quantifying and visualising the nuances of cellular dynamics in vivo using intravital imaging

Kendelle J Murphy; Daniel A Reed; Michael Trpceski; David Herrmann; Paul Timpson

doi:10.1016/j.ceb.2021.04.007

Quantifying and visualising the nuances of cellular dynamics in vivo using intravital imaging

Curr Opin Cell Biol. 2021 Oct:72:41-53. doi: 10.1016/j.ceb.2021.04.007. Epub 2021 Jun 3.

Authors

Kendelle J Murphy¹, Daniel A Reed¹, Michael Trpceski², David Herrmann³, Paul Timpson⁴

Affiliations

¹ Garvan Institute of Medical Research & The Kinghorn Cancer Centre, Cancer Theme, Sydney, NSW, 2010, Australia; St Vincent's Clinical School, Faculty of Medicine, UNSW Sydney, Sydney, NSW, 2010, Australia.
² Garvan Institute of Medical Research & The Kinghorn Cancer Centre, Cancer Theme, Sydney, NSW, 2010, Australia.
³ Garvan Institute of Medical Research & The Kinghorn Cancer Centre, Cancer Theme, Sydney, NSW, 2010, Australia; St Vincent's Clinical School, Faculty of Medicine, UNSW Sydney, Sydney, NSW, 2010, Australia. Electronic address: [email protected].
⁴ Garvan Institute of Medical Research & The Kinghorn Cancer Centre, Cancer Theme, Sydney, NSW, 2010, Australia; St Vincent's Clinical School, Faculty of Medicine, UNSW Sydney, Sydney, NSW, 2010, Australia. Electronic address: [email protected].

PMID: 34091131
DOI: 10.1016/j.ceb.2021.04.007

Abstract

Intravital imaging is a powerful technology used to quantify and track dynamic changes in live cells and tissues within an intact environment. The ability to watch cell biology in real-time 'as it happens' has provided novel insight into tissue homeostasis, as well as disease initiation, progression and response to treatment. In this minireview, we highlight recent advances in the field of intravital microscopy, touching upon advances in awake versus anaesthesia-based approaches, as well as the integration of biosensors into intravital imaging. We also discuss current challenges that, in our opinion, need to be overcome to further advance the field of intravital imaging at the single-cell, subcellular and molecular resolution to reveal nuances of cell behaviour that can be targeted in complex disease settings.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Intravital Microscopy*