Comparison of the Acinetobacter baumannii Reference Strains ATCC 17978 and ATCC 19606 in Antimicrobial Resistance Mediated by the AdeABC Efflux Pump

K Lucaßen; S Gerson; K Xanthopoulou; J Wille; T Wille; H Seifert; P G Higgins

doi:10.1128/AAC.00570-21

Comparison of the Acinetobacter baumannii Reference Strains ATCC 17978 and ATCC 19606 in Antimicrobial Resistance Mediated by the AdeABC Efflux Pump

Antimicrob Agents Chemother. 2021 Jul 16;65(8):e0057021. doi: 10.1128/AAC.00570-21. Epub 2021 Jul 16.

Authors

K Lucaßen¹, S Gerson¹, K Xanthopoulou^{1

2}, J Wille^{1

2}, T Wille¹, H Seifert^{1

2}, P G Higgins^{1

2}

Affiliations

¹ Institute for Medical Microbiology, Immunology and Hygiene, University of Cologne, Cologne, Germany.
² German Center for Infection Research (DZIF), Partner Site Bonn-Cologne, Cologne, Germany.

Abstract

The Acinetobacter baumannii RND efflux pump AdeABC is regulated by the 2-component regulator AdeRS. In this study, we compared the regulation and expression of AdeABC of the reference strains ATCC 17978 and ATCC 19606. A clearly stronger efflux activity was demonstrated for ATCC 19606. An amino acid substitution at residue 172 of adeS was identified as a potential cause for differential expression of the pump. Therefore, we recommend caution with exclusively using single reference strains for research.

Keywords: Acinetobacter; antibiotic resistance; cloning; efflux pumps.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Acinetobacter baumannii* / genetics
Acinetobacter baumannii* / metabolism
Anti-Bacterial Agents / pharmacology
Bacterial Proteins / genetics
Bacterial Proteins / metabolism
Drug Resistance, Bacterial / genetics
Drug Resistance, Multiple, Bacterial / genetics
Membrane Transport Proteins / genetics
Microbial Sensitivity Tests

Substances

Anti-Bacterial Agents
Bacterial Proteins
Membrane Transport Proteins