Introduction: The occurrence of Microtriatoma borbai in the state of Espírito Santo, Brazil is reported by the first time.
Methods: A triatomine specimen collected in a hybrid eucalyptus crop in the municipality of Aracruz, Espírito Santo state was found to be a male M. borbai.
Results: This finding expands the geographical distribution of M. borbai from four to five Brazilian states. It is the first report of M. borbai occurrence inside a eucalyptus crop.
Conclusions: The occurrence of M. borbai in the state of Espírito Santo broadens the geographical distribution of this species in southeastern Brazil.