Synergistic ammonia and fatty acids inhibition of microbial communities during slaughterhouse waste digestion for biogas production

Zhaodi Guo; Muhammad Usman; Saeed A Alsareii; Farid A Harraz; M S Al-Assiri; Mohammed Jalalah; Xiangkai Li; El-Sayed Salama

doi:10.1016/j.biortech.2021.125383

Synergistic ammonia and fatty acids inhibition of microbial communities during slaughterhouse waste digestion for biogas production

Bioresour Technol. 2021 Oct:337:125383. doi: 10.1016/j.biortech.2021.125383. Epub 2021 Jun 7.

Authors

Zhaodi Guo¹, Muhammad Usman¹, Saeed A Alsareii², Farid A Harraz³, M S Al-Assiri⁴, Mohammed Jalalah⁵, Xiangkai Li⁶, El-Sayed Salama⁷

Affiliations

¹ Department of Occupational and Environmental Health, School of Public Health, Lanzhou University, Lanzhou 730000, Gansu Province, PR China.
² Promising Centre for Sensors and Electronic Devices (PCSED), Advanced Materials and Nano-Research Centre, Najran University, P.O. Box: 1988, Najran 11001, Saudi Arabia; Department of Surgery, College of Medicine, Najran University, Najran, Saudi Arabia.
³ Promising Centre for Sensors and Electronic Devices (PCSED), Advanced Materials and Nano-Research Centre, Najran University, P.O. Box: 1988, Najran 11001, Saudi Arabia; Nanomaterials and Nanotechnology Department, Central Metallurgical Research and Development Institute (CMRDI), P.O. 87, Helwan, Cairo 11421, Egypt.
⁴ Promising Centre for Sensors and Electronic Devices (PCSED), Advanced Materials and Nano-Research Centre, Najran University, P.O. Box: 1988, Najran 11001, Saudi Arabia.
⁵ Promising Centre for Sensors and Electronic Devices (PCSED), Advanced Materials and Nano-Research Centre, Najran University, P.O. Box: 1988, Najran 11001, Saudi Arabia; Department of Electrical Engineering, Faculty of Engineering, Najran University, P.O. Box: 1988, Najran 11001, Saudi Arabia.
⁶ MOE, Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, Lanzhou, Gansu 730000, PR China.
⁷ Department of Occupational and Environmental Health, School of Public Health, Lanzhou University, Lanzhou 730000, Gansu Province, PR China. Electronic address: [email protected].

PMID: 34126358
DOI: 10.1016/j.biortech.2021.125383

Abstract

The slaughterhouse waste (SHW) contains high organics which makes SHW a feasible feedstock for anaerobic digestion (AD). The present study systematically assessed the microbiome response and biomethanation along with the production of volatile fatty acids (VFAs) and ammonia under 2%, 4%, 6%, and 8% (w v^-1) loadings of SHW in AD. The optimum loading was 2% SHW which resulted in maximum biomethane production and VFAs consumption. A higher SHW concentration (4% and 6%) resulted in a prolonged lag-phase and decreased biomethane production. High VFAs (28.88 g L^-1) and ammonia nitrogen (>4 g L^-1) accumulation were observed at 8% SHW leading to permanent inhibition of biomethane and methanogenic archaea. An increase in ammonia and VFAs concentration, at 4% and 6% SHW loadings, shifted the methanogenic pathway from acetoclastic to hydrogenotrophic lead by Methanoculleus. Acetoclastic Methanosaeta (77.15%) dominated the reactors loaded with 2% SHW resulting in the highest biomethane production.

Keywords: Ammonia; Anaerobic digestion; Fatty acids; Microbiome response; Slaughterhouse waste.

MeSH terms

Abattoirs
Ammonia*
Anaerobiosis
Biofuels
Bioreactors
Digestion
Fatty Acids
Methane
Microbiota*

Substances

Biofuels
Fatty Acids
Ammonia
Methane