Interaction of a PDZ Protein Domain with a Synthetic Library of All Human Protein C Termini

Ulrich Hoffmüller; Michael Russwurm; Frank Kleinjung; Jennifer Ashurst; Hartmut Oschkinat; Rudolf Volkmer-Engert; Doris Koesling; Jens Schneider-Mergener

doi:10.1002/(SICI)1521-3773(19990712)38:13/14<2000::AID-ANIE2000>3.0.CO;2-V

Interaction of a PDZ Protein Domain with a Synthetic Library of All Human Protein C Termini

Angew Chem Int Ed Engl. 1999 Jul 12;38(13-14):2000-2004. doi: 10.1002/(SICI)1521-3773(19990712)38:13/14<2000::AID-ANIE2000>3.0.CO;2-V.

Authors

Ulrich Hoffmüller¹, Michael Russwurm², Frank Kleinjung³, Jennifer Ashurst⁴, Hartmut Oschkinat⁵, Rudolf Volkmer-Engert¹, Doris Koesling², Jens Schneider-Mergener¹

Affiliations

¹ Institut für Medizinische Immunologie, Universitätsklinikum Charité, Humboldt-Universität Berlin, Schumannstrasse 20/21, D-10098 Berlin (Germany), Fax: (+49) 30-2802-6460.
² Institut für Pharmakologie, Freie Universität Berlin, Thielallee 69-73, D-14195 Berlin (Germany).
³ Institut für Analytische Biochemie, Universität Potsdam, D-14943 Luckenwalde (Germany).
⁴ Department of Plant Sciences, University of Cambridge, Downing St., Cambridge CB2 3EA (UK).
⁵ Forschungsinstitut für Molekulare Pharmakologie, Alfred-Kowalke-Strasse 4, D-10315 Berlin (Germany).

Abstract

New interaction partners of a PDZ protein domain were identified through use of a library made up of all known human protein C-terminal peptides ("P" in the schematic representation). The library was displayed on a cellulose membrane by positional resolution of inverted peptides.

Keywords: Molecular recognition; Peptides; Peptide–peptide interactions.